In vitro and in silico antimicrobial activity of sterol and flavonoid isolated from Trianthema decandra L

Phytochemical study on the leaves of Trianthema decandra leads to the isolation and characterization of two compounds from chloroform extract. The compounds were characterized using HPLC, UV, FT-IR, NMR, LCMS and CHNS. The structure of compounds were elucidated from spectral data and named according to rules laid down in IUPAC nomenclature. A novel sterol was named as 17-(5-ethyl-6-methylheptan-2-yl) -4, 4, 10, 13-tetramethyl-hexadecahydro-1H-cyclopenta (alpha) phenanthren-3-ol and the flavonoid was named as 2-(3, 4 dihydroxy - phenyl)-3, 5, 7 - trihydroxy-chromen-4 one. The antimicrobial activity of sterol and flavonoid isolated from T. decandra were examined by disc diffusion and broth dilution assays. The isolated compounds showed excellent activity against all the tested microorganisms. Sterol exhibits MIC value of 39.05 mu g/ml against S. typhi whereas flavonoid shows 78.10 mu g/ml against V. cholerae. The plausible mode of action of these compounds was studied using in silica molecular docking with Penicillin Binding Protein (PBP) as target.