Journal
METHODS
Volume 136, Issue -, Pages 116-125Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2017.10.004
Keywords
Quantitative phase imaging; Optofluidic time-stretch microscopy; High-throughput screening; Single-cell analysis; Machine learning; Microfluidics
Funding
- ImPACT Program of the CSTI (Cabinet Office, Government of Japan)
- Noguchi Shitagau Research Grant
- New Technology Development Foundation
- Konica Minolta Imaging Science Encouragement Award
- JSPS KAKENHI [25702024, 25560190]
- JGC-S Scholarship Foundation
- Mitsubishi Foundation
- TOBIRA Award
- Takeda Science Foundation
- Burroughs Wellcome Foundation
- MEXT, Japan
- Grants-in-Aid for Scientific Research [25702024, 25560190, 15J02613] Funding Source: KAKEN
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Innovations in optical microscopy have opened new windows onto scientific research, industrial quality control, and medical practice over the last few decades. One of such innovations is optofluidic time stretch quantitative phase microscopy - an emerging method for high-throughput quantitative phase imaging that builds on the interference between temporally stretched signal and reference pulses by using dispersive properties of light in both spatial and temporal domains in an interferometric configuration on a microfluidic platform. It achieves the continuous acquisition of both intensity and phase images with a high throughput of more than 10,000 particles or cells per second by overcoming speed limitations that exist in conventional quantitative phase imaging methods. Applications enabled by such capabilities are versatile and include characterization of cancer cells and microalgal cultures. In this paper, we review the principles and applications of optofluidic time-stretch quantitative phase microscopy and discuss its future perspective. (C) 2017 Elsevier Inc. All rights reserved.
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