Journal
METHODS
Volume 142, Issue -, Pages 16-23Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2018.04.008
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Funding
- TRI-Imaging platform Toulouse
- Ligue Nationale Contre le Cancer
- Agence Nationale de la Recherche (ANR)
- Fondation Association pour la Recherche sur le Cancer (ARC)
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Spatio-temporal organization of the cell nucleus adapts to and regulates genomic processes. Microscopy approaches that enable direct monitoring of specific chromatin sites in single cells and in real time are needed to better understand the dynamics involved. In this chapter, we describe the principle and development of ANCHOR, a novel tool for DNA labelling in eukaryotic cells. Protocols for use of ANCHOR to visualize a single genomic locus in eukaryotic cells are presented. We describe an approach for live cell imaging of a DNA locus during the entire cell cycle in human breast cancer cells.
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