Journal
METABOLIC ENGINEERING
Volume 48, Issue -, Pages 63-71Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2018.05.011
Keywords
Beta-reduction; Reverse beta oxidation; Fatty alcohol; Medium chain alcohol; Anaerobic; E. coli; Oleochemical; Acyl-CoA reductase
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Funding
- Dow Chemical Company
- NIH, NHGRI Genomic Sciences Training Program [T32 HG002760]
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In this report, we identify the relevant factors to increase production of medium chain n-alcohols through an expanded view of the reverse beta-oxidation pathway. We began by creating a base strain capable of producing medium chain n-alcohols from glucose using a redox-balanced and growth-coupled metabolic engineering strategy. By dividing the heterologous enzymes in the pathway into different modules, we were able to identify and evaluate homologs of each enzyme within the pathway and identify several capable of enhancing medium chain alcohol titers and/or selectivity. In general, the identity of the trans-2-enoyl-CoA reductase (TER) and the direct overexpression of the thiolase (FadA) and beta-hydroxy-acyl-CoA reductase (FadB) improved alcohol titer and the identity of the FadBA complex influenced the dominant chain length. Next, we linked the anaerobically induced VHb promoter from Vitreoscilla hemoglobin to each gene to remove the need for chemical inducers and ensure robust expression. The highest performing strain with the autoinduced reverse beta-oxidation pathway produced n-alcohols at titers of 1.8 g/L with an apparent molar yield of 0.2 on glucose consumed in rich medium (52% of theoretical yield).
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