Journal
METABOLIC ENGINEERING
Volume 50, Issue -, Pages 85-108Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2018.04.011
Keywords
Metabolic engineering; Pathway optimization; Genome engineering; Saccharomyces cerevisiae
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Funding
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0018420, DE-SC0018260]
- University of Illinois at Urbana-Champaign
- Fundamental Research Funds for the Central Universities [2018QNA4039]
- Zhejiang University
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Metabolic engineering aims to develop efficient cell factories by rewiring cellular metabolism. As one of the most commonly used cell factories, Saccharomyces cerevisiae has been extensively engineered to produce a wide variety of products at high levels from various feedstocks. In this review, we summarize the recent development of metabolic engineering approaches to modulate yeast metabolism with representative examples. Particularly, we highlight new tools for biosynthetic pathway optimization (i.e. combinatorial transcriptional engineering and dynamic metabolic flux control) and genome engineering (i.e. clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated (Cas) system based genome engineering and RNA interference assisted genome evolution) to advance metabolic engineering in yeast. We also discuss the challenges and perspectives for high throughput metabolic engineering.
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