Journal
STEM CELL NICHE: METHODS AND PROTOCOLS
Volume 1035, Issue -, Pages 217-230Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-62703-508-8_19
Keywords
Acute myeloid leukemia; Leukemia stem cells; Hematopoietic stem cells; Flow cytometry; FACS; Aldehyde dehydrogenase; Aberrant markers
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Mounting evidences indicate that leukemic cells in patients with acute myeloid leukemia (AML) are derived from leukemia stem cells (LSC). In analogy to normal hematopoietic stem cells (HSC), LSC remain mostly dormant and are hence resistant to conventional chemotherapy. Residual, physiological HSC exist alongside with LSC, with heterogeneous dominance of LSC over HSC in individual patients. We have devised a flow cytometric method for the identification and separation of these two stem cell populations based on surface antigen markers such as CD34, CD38, lineage aberrant markers, and aldehyde dehydrogenase (ALDH) enzyme activity.
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