4.6 Article

Culturing functional pancreatic islets on α5-Iaminins and curative transplantation , to diabetic mice

Journal

MATRIX BIOLOGY
Volume 70, Issue -, Pages 5-19

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.matbio.2018.03.018

Keywords

Diabetes; Laminin; Pancreatic; Islet; Transplantation; beta-cell

Funding

  1. National Medical Research Council, Singapore [NMRC/STaR/010/2012, CBRG15may062, WBS R913200076263]
  2. Swedish Cancer Society
  3. Vetenskapsradet, Sweden
  4. Diabetesfonden, Sweden
  5. Diabetes Research & Wellness Foundation, Sweden [2749/2014SW]

Ask authors/readers for more resources

The efficacy of islet transplantation for diabetes treatment suffers from lack of cadaver-derived islets, islet necrosis and long transfer times prior to transplantation. Here, we developed a method for culturing mouse and human islets in vitro on alpha 5-laminins, which are natural components of islet basement membranes. Adhering islets spread to form layers of 1-3 cells in thickness and remained normoxic and functional for at least 7 days in culture. In contrast, spherical islets kept in suspension developed hypoxia and central necrosis within 16 h. Transplantation of 110-150 mouse islets cultured on alpha 5-laminin-coated polydimethylsiloxane membranes for 3-7 days normalized blood glucose already within 3 days in mice with streptozotocin-induced diabetes. RNA-sequencing of isolated and cultured mouse islets provided further evidence for the adhesion and spreading achieved with alpha 5-laminin. Our results suggest that use of such in vitro expanded islets may significantly enhance the efficacy of islet transplantation treatment for diabetes. (C) 2018 The Authors. Published by Elsevier B.V.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available