Journal
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 90, Issue -, Pages 280-288Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2018.04.080
Keywords
Polycaprolcatone; Bioactive glass; Bone tissue engineering scaffold; Elastic and viscoelastic mechanical properties; In vitro assay; In vivo study
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Microsphere sintering method was used to fabricate bone tissue engineering scaffolds made of polycaproiactone (PCL)/bioactive glass 58S5Z (58S modified with 5 wt% Zinc). First, the effect of PCL/58S5Z ratio on the mechanical properties (elastic modulus and yield strength) was investigated. It was found that samples with 5 wt% 58S5Z (named 5%BG) had the highest elastic modulus and yield strength among all samples, i.e., with 0, 5, 10, and 20 wt% bioactive glass. Then, considering the importance of viscoelastic properties of bone, the viscoelastic behavior of 0%BG (scaffold with only PCL) and 5%BG samples was determined by performing compressive stress relaxation test and subsequently a Generalized Maxwell model was developed. Findings indicated a similar amount and pattern of predicted storage and loss moduli and loss factor of the composite scaffolds to those of the bone. In the next step, the analysis of biological behavior of the scaffolds using MTT assay, DAPI and Alizarin red staining demonstrated that 5%BG scaffolds had higher in vitro cell viability and bone formation compared to 0% BG ones. Furthermore, in vivo study employing H&E staining of the scaffolds implanted in rate calvarium for 50 days, confirmed the earlier findings and showed that 5%BG-filled defects had higher and more uniform bone formation compared to both 0%BG-filled and empty defects.
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