Journal
FLUIDS AND BARRIERS OF THE CNS
Volume 10, Issue -, Pages -Publisher
BMC
DOI: 10.1186/2045-8118-10-35
Keywords
Cerebrospinal fluid; Lymphatic; Nanotechnology; In vivo imaging; Mouse; Hyperspectral imaging; Non-invasive imaging; Quantum dot
Categories
Funding
- Canadian Institutes of Health Research [MOP119432]
- Glaucoma Research Society of Canada
- Dorothy Pitts Research Fund
- Thor and Nicky Eaton Research Fund
- Henry Farrugia Research Fund
- National Science and Engineering Research Council CGS Award
- Vision Science Research Program Award
Ask authors/readers for more resources
Background: Mouse models are commonly used to study central nervous system disorders, in which cerebrospinal fluid (CSF) drainage may be disturbed. However, mouse CSF drainage into lymphatics has not been thoroughly characterized. We aimed to image this using an in vivo approach that combined quantum dot fluorescent nanoparticles with hyperspectral imaging. Findings: Quantum dot 655 was injected into the CSF of the cisterna magna in seven mice and visualized by in vivo hyperspectral imaging at time points 20 and 40 min, 1, 2, and 6 h after injection. In controls (n = 4), quantum dots were applied directly onto intact dura mater covering the cisterna magna. After imaging, lymph nodes in the neck were harvested and processed post-mortem for histological analysis. After injection into the CSF, quantum dot signal was detected in vivo in submandibular lymph nodes of all mice studied as early as 20 min, but not in controls. Post-mortem gross and histological examination of lymph nodes confirmed in vivo observations. \Conclusions: Non-invasive in vivo hyperspectral imaging is a useful tool to study CSF lymphatic drainage and is relevant to understanding this pathway in CNS disease models.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available