Journal
LASERS IN MEDICAL SCIENCE
Volume 33, Issue 7, Pages 1455-1460Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s10103-018-2490-0
Keywords
Photodynamic antimicrobial chemotherapy (PACT); Pseudomonas aeruginosa; Chlorin derivative; TONS 504; Light-emitting diode (LED); Infectious keratitis
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Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [15 K10894]
- Grants-in-Aid for Scientific Research [15K10894] Funding Source: KAKEN
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Pseudomonas aeruginosa (P. aeruginosa) is a major cause of infectious keratitis, which itself is a major cause of blindness worldwide. We have now evaluated the time-dependent effectiveness of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS 504 and a light-emitting diode (LED) on P aeruginosa in vitro. PACT with TONS 504 (10 mg/L) and irradiation (30 J/m(2)) by an LED device that delivers light centered on a wavelength of 660 nm was applied to 1 x 10(6) colony-forming units of P. aeruginosa in liquid medium. The bacteria were then cultured at 37 degrees C for various times before assay of viability by determination of colony formation on agar plates. The effect of a second irradiation at 3 h after the initial LED exposure was also examined. Bacterial growth was markedly inhibited between 3 and 9 h after PACT with TONS 504, with the maximal effect being apparent at 3 h. Furthermore, a second exposure to LED irradiation at 3 h after the first treatment enhanced the inhibitory effect on bacterial growth. PACT with TONS 504 thus inhibited the growth of P. aeruginosa in a time-dependent manner, and an additional irradiation exposure applied 3 h after the first LED treatment greatly increased the effectiveness of PACT. This antibacterial system thus warrants further evaluation with regard to its potential effectiveness for the treatment of infectious keratitis.
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