Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 252, Issue -, Pages 80-85Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2017.11.010
Keywords
Apple chlorotic leaf spot virus; Apple stem pitting virus; Loop-mediated isothermal amplification; Pear; Rt-per
Funding
- Chinese Ministry of Agriculture [nyhyzx-201203076-03, CARS-29-10]
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A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Apple chlorotic leaf spot virus (ACLSV) and Apple stem pitting virus (ASPV), two important viruses frequently occurring in pear trees. A set of four RT-LAMP primers designed based on the highly conserved region of each CP gene of the two viruses showed high specificity and feasibility for ACLSV and ASPV detections. The RT-LAMP assays for ACLSV and ASPV in pear samples were 10(4) and 10(3) times more sensitive than that of conventional RTPCR assays. The RT-LAMP under optimal reaction condition was subsequently utilized in the detection of the two viruses in-vitro cultures of pear and field pear samples. This study provides a rapid and sensitive tool to determine the infection statues of the two viruses in pear certification program.
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