Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 80, Issue 1, Pages 98-102Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.17-0406
Keywords
Asia; Fasciola; hybrid; pepck; pold
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23405044, 24580420]
- Grants-in-Aid for Scientific Research [24580420, 16H05806, 16J02164] Funding Source: KAKEN
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Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F. hepatica and F. gigantica (mixed types) in pepck and/or pold. These findings provide clear evidence for the hybrid origin of aspermic Fasciola lineages and suggest that aspermic Fasciola flukes should hereafter be called hybrid Fasciola flukes.
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