Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 80, Issue 2, Pages 205-212Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.17-0528
Keywords
colon; heat-stable enterotoxin A; piglet; recombinant escherichia coli
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Funding
- Hubei Provincial Technology and Innovation Program [2017AHB062]
- National Key Research and Development Program of China [2017YFD0500505]
- National Natural Science Foundation of China [31302089]
- Agriculture and Agri-Food Canada A-base project [J-001391]
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Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 x 10(9) CFU E. coli LMG194-STa), LMG194 group (2 x 10(9) CFU E. coli LMG194) and K88 group (2 x 10(9) CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H2O2; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines.
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