Journal
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
Volume 48, Issue -, Pages 1-7Publisher
ELSEVIER GMBH
DOI: 10.1016/j.jtemb.2018.02.022
Keywords
Ascorbic acid; Vitamin C; Chromitun(Cr6+); Nephrotoxicity; Apoptosis; Autophagy
Funding
- Kaohsiung Medical University Hospital [KMUH99-9R25]
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Chromium poisoning can cause renal failure and death. Chromium intoxication may be managed using L-ascorbic acid (vitamin C) therapy. However, the evidence supporting the effectiveness of this treatment is insufficient, and the mechanism of action has not been clarified in renal cells. In this study, our results showed that the optimal regimen of L-ascorbic acid therapy in human epithelial renal proximal tubule cells, HK-2 cells, was 30 g/mL. Supplementation of L-ascorbic acid with 30 mu g/mL and within 8 h of chromium intoxication (K2Cr2O7, Cr6+) was effective to inhibit renal tubular cell damage by blocking generation of free radicals, cell apoptosis, and autophagy. Intracellular chromium concentrations were estimated using electrothermal atomic absorption spectrometry. Treatment of L-ascorbic acid within 8 h of chromium intoxication significantly decreased the entry of chromium into the cells. Moreover, concomitant administration of L-ascorbic acid with repeatedly dosing at 8 hourly intervals had a better protective effect at lower concentration of L-ascorbic acid when compared to single dosing of L-ascorbic acid at an early time point of chromium intoxication. These findings might help physicians develop effective therapy strategies in renal failure.
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