Journal
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
Volume 15, Issue 10, Pages 2295-2305Publisher
SPRINGER
DOI: 10.1007/s13738-018-1418-8
Keywords
Retention indices; Correspondence factor analysis; Solvation parameter model; Monosubstituted benzenes; Characterization of RPLC stationary phases; Retention mechanism
Categories
Ask authors/readers for more resources
In reversed-phase liquid chromatography (RPLC), the comparison of experimental results obtained from different columns is a complex problem. A correspondence factor analysis (CFA) and a linear solvation energy relationship (LSER) were applied on retention data to characterize second-order intermolecular interactions responsible for retention on a set of RPLC columns. Seven octadecyl-C-18 columns with different packing materials are obtained from different manufacturers and one octyl-C-8 column. The retention data were determined under isocratic conditions using a methanol-water (65:35, v/v) mobile phase. The chromatographic retention indices based on alkan-2-ones and alkyl aryl ketones retention index scales are calculated using a multiparametric least-squares regressions iterative method. The CFA and LSER results permitted to highlight that the retention indices were appropriate for studying the second-order retention mechanisms on the eight chromatographic systems investigated and exhibited the best reproducibility. Although many earlier studies have reported the use of chemometric methods to characterize chemical factors affecting retention in RPLC using retention factors as retention parameters, this is the first study based on retention indices.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available