Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 202, Issue 2, Pages 161-169Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2018.01.002
Keywords
cryoEM vitrification; piezo dispensing; nanowire grids
Funding
- NIH [GM103310, OD019994]
- Simons Foundation [SF349247]
Ask authors/readers for more resources
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to similar to 3 angstrom resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available