Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 202, Issue 2, Pages 170-174Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2018.01.001
Keywords
cryoEM vitrification; Spotiton; Nanowire grids; TEM grids
Funding
- NIH [GM103310, OD019994]
- Simons Foundation [349247]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103310] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [S10OD019994] Funding Source: NIH RePORTER
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We have developed a self-blotting TEM grid for use with a novel instrument for vitrifying samples for cryo-electron microscopy (cryoEM). Nanowires are grown on the copper surface of the grid using a simple chemical reaction and the opposite smooth side is used to adhere to a holey sample substrate support, for example carbon or gold. When small volumes of sample are applied to the nanowire grids the wires effectively act as blotting paper to rapidly wick away the liquid, leaving behind a thin film. In this technical note, we present a detailed description of how we make these grids using a variety of substrates fenestrated with either lacey or regularly spaced holes. We explain how we characterize the quality of the grids and we describe their behavior under a variety of conditions.
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