Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 203, Issue 3, Pages 230-235Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2018.05.014
Keywords
Cryo-electron microscopy; Cloud computing; Amazon Web Services
Funding
- National Institutes of Health [R01GM107214, R01GM123089]
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Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools (cryoem-cloud-tools) have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2 angstrom structure of beta-galactosidase in similar to 55 h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud.
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