Journal
JOURNAL OF SEPARATION SCIENCE
Volume 41, Issue 12, Pages 2623-2631Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201701432
Keywords
capacitively coupled contactless conductivity; capillary electrophoresis; citrate; metabolism; oxalate
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Funding
- National Natural Science Foundation of China [21475119, 21275133]
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A new method for the rapid determination of the metabolites oxalate and citrate in urine samples was based on capillary electrophoresis and capacitively coupled contactless conductivity detection coupled with solid-phase extraction. The detection cell for capacitively coupled contactless conductivity detection was improved with a smaller inner volume (1.5 nL), reduced noise (0.2 similar to 0.5 mV) and better reproducibility and durability. Under optimal conditions, oxalate and citrate can achieve baseline separation within 4 min and the detection limits (S/N = 3) for oxalate and citrate are about 44 and 244 ng/mL, respectively. The overall recovery is between 80.0 and 89.2%. This method offers a better choice for quantitative analysis of strong anions such as oxalate and citrate in diagnostic testing associated with human diseases.
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