Journal
JOURNAL OF SEPARATION SCIENCE
Volume 41, Issue 11, Pages 2321-2330Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201701439
Keywords
isoflavonoids; microdialysis; neurotransmitters; tandem mass spectrometry; traditional Chinese medicine
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Funding
- Hospital Start-up Scientific Research Funds for the Dr. Scholars [BS15013]
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Pueraria lobata is a medicinal plant widely used in traditional Chinese medicine. The total pueraria isoflavones have demonstrated positive effect against neurological disorders. In the present study, we first develop an ultra high performance liquid chromatography and tandem mass spectrometry method to quantify the multiple active pueraria isoflavonoids and neurochemical markers in brain dialysate to provide tools for further exploring the functional mechanism of pueraria isoflavones for neuroactivities. A phenomenex Luna C18 column (50 x 2.0 mm, 5 mu m) was employed with acetonitrile/0.05% formic acid in water as the mobile phase for the separation of analytes. Amass spectrometer with electrospray ionization source in positive/negative ion switching mode was used for multiple reaction monitoring of the detected compounds. The method was validated and proved acceptable. The intra-and interday precision across quality control levels was within 13.87 for all analytes, whereas the deviation of assay accuracies ranged between 0.03 and 11.53%. The method was successfully applied to a pharmacological study of pueraria isoflavones in rat brain.
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