Journal
JOURNAL OF SEPARATION SCIENCE
Volume 41, Issue 15, Pages 3113-3120Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201800183
Keywords
anticancer drugs; automated liquid-phase microextraction; high-performance liquid chromatography; hollow fibers; immiscible organic solvents
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Funding
- Research Council and Graduate School of Tarbiat Modares University (Tehran, Iran)
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An automated three-phase hollow fiber liquid-phase microextraction based on two immiscible organic solvents followed by high-performance liquid chromatography with UV-Vis detection method was applied for the extraction and determination of exemestane, letrozole, and paclitaxel in water and urine samples. n-Dodecane was selected as the supported liquid membrane and its polarity was justified by trioctylphosphine oxide. Acetonitrile was used as an organic acceptor phase with desirable immiscibility having n-dodecane. All the effective parameters of the microextraction procedure such as type of the organic acceptor phase, the supported liquid membrane composition, extraction time, pH of the donor phase, hollow fiber length, stirring rate, and ionic strength were evaluated and optimized separately by a one variable at-a-time method. Under the optimal conditions, the linear dynamic ranges were 1.8-200 (R-2 = 0.9991), 0.9-200 (R-2 = 0.9987) and 1.2-200 mu g/L (R-2 = 0.9983), and the limits of detection were 0.6, 0.3, and 0.4 mu g/L for exemestane, letrozole, and paclitaxel, respectively. To evaluate the capability of the proposed method in the analysis of biological samples, three different urinary samples were analyzed under the optimal conditions. The relative recoveries of the three pharmaceuticals were in the range of 91-107.3% for these three analytes.
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