Journal
JOURNAL OF PROSTHETIC DENTISTRY
Volume 121, Issue 1, Pages 135-142Publisher
MOSBY-ELSEVIER
DOI: 10.1016/j.prosdent.2018.02.005
Keywords
-
Categories
Funding
- Sao Paulo Research Foundation-FAPESP [2012/11074-2, 2012/24291-1, 2013/10400-6]
- National Council for the Improvement of Higher Education-CAPES
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [12/11074-2] Funding Source: FAPESP
Ask authors/readers for more resources
Statement of problem. Antifungal agents incorporated into interim denture resilient liners have been suggested as an adjunct treatment for denture stomatitis (DS). However, before applying this protocol to humans, biocompatibility analysis of such drugs in animal models is required. Purpose. The purpose of this animal study was to evaluate the in vivo biocompatibility of an interim resilient liner modified with minimum inhibitory concentrations (MICs) of antifungal drugs for Candida albicans biofilm. Materials and methods. Sixty Wistar rats were divided into 6 groups (n=5): PC=positive control/no protocol; IOD (intraoral device)=rats using an acrylic resin palatal device (PD); Tru=rats using a PD relined with Trusoft; and Ny (nystatin), Chx (chlorhexidine diacetate), and Ke (ketoconazole) groups=rats using a PD relined with Trusoft + drug MICs. The rats were sacrificed at 7 or 14 days of trial. Histopathological qualitative analysis was performed by comparing photomicrographs of histological sections of the intermolar region. Morphological changes in the epithelium and keratin were quantitatively analyzed by computerized planimetry, and data were analyzed by using 2-way ANOVA and the Tukey HSD test (alpha=.05). Results. Quantitative analysis showed that only PD containing Ke significantly decreased the thickness and area of the keratin compared with the other groups (P<.001), which showed no differences between each other (P>.05). These results agreed with those of qualitative analysis. Conclusion. Incorporation of MICs of Ny and Chx in Trusoft did not induce histopathological changes in the rat palatal mucosa, suggesting the in vivo biocompatibility of this DS treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available