Journal
JOURNAL OF PINEAL RESEARCH
Volume 65, Issue 1, Pages -Publisher
WILEY
DOI: 10.1111/jpi.12483
Keywords
chondrogenesis; human mesenchymal stem cells; melatonin; miR-526b-3p; miR-590-5p; SMAD7
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Funding
- Natural Science Basic Research Plan in Shanxi Province of China [2017JQ8056]
- China Postdoctoral Science Foundation [2017M613177]
- Natural Science Foundation of Guangdong Province, China [2016A030313284, 2017A030311008]
- National Natural Science Foundation of China [81371907, 81572134, 81601898]
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Bone marrow-derived mesenchymal stem cells (BMSCs), with inherent chondrogenic differentiation potential appear to be ideally suited for therapeutic use in cartilage regeneration. Accumulating evidence has demonstrated that melatonin can promote chondrogenic differentiation in human BMSCs. However, little is known about the mechanism. MicroRNAs (miRNAs) have been shown to regulate the differentiation of BMSCs, but their roles in melatonin-promoted chondrogenic differentiation have not been characterized. Here, we demonstrate that melatonin promoted chondrogenic differentiation of human BMSCs via upregulation of miR-526b-3p and miR-590-5p. Mechanistically, the elevated miR-526b-3p and miR-590-5p enhanced SMAD1 phosphorylation by targeting SMAD7. Additionally, administration of miR-526b-3p mimics or miR-590-5p mimics successfully promoted the chondrogenic differentiation of human BMSCs. Collectively, our study suggests that modification of BMSCs using melatonin or miRNA transduction could be an effective therapy for cartilage damage and degeneration.
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