Journal
BIOLOGIA
Volume 69, Issue 3, Pages 341-349Publisher
SPRINGER
DOI: 10.2478/s11756-013-0322-z
Keywords
antioxidant; callus; DPPH assay; free radical scavenging; micropropagation; phenolics
Categories
Funding
- Higher Education Commission of Pakistan
- Quaid-i-Azam University, Islamabad, Pakistan
Ask authors/readers for more resources
Present study describes rapid in vitro propagation of Caralluma tuberculata, a traditional medicinal plant, and antioxidant potential of calli and plants extracts. The highest callus induction rate (93.3%) with maximum weight of calli 5.2 g was achieved from shoot tip explants on MS medium supplemented with 9.04 mu M 2,4-D and 4.44 mu M BA. The maximum shoot induction rate (71.1%) with mean number of shoots 3.66 +/- 1.53 and 4.6 cm average shoot length was observed on 13.32 mu M BA, 4.52 mu M 2,4-D and 2.89 mu M GA(3) appended in MS medium. The developed shoots were best rooted in the presence of 5.07 mu M IAA with 3.0 +/- 0.15 roots per plantlet. The plants were successfully acclimatized under in vivo conditions. The plants and calli extracts exhibited good antioxidant activities, however, plant extract activities were more pronounced. The phenolic compounds in plant and calli extracts were 0.16% and 0.057%, respectively. While the flavonoids were 0.092% in plant and 0.039% in calli extract. Total Phenolics, flavonoids; DPPH radical scavenging activity and reducing power potential distributed among different fractions depending upon polarity of the solvent. The highest DPPH scavenging activity and reducing power was exhibited by water fractions; 4.95 mg/mL and 0.729 OD at 10 mg/mL, respectively. The micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant. Furthermore, antioxidant value describes importance of this valuable plant as food and medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available