Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 159, Issue -, Pages 252-261Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2018.06.065
Keywords
Corydalis saxicola bunting; Liver fibrosis; Metabonomics(1)H-NMR; Network pharmacology; Carbon tetrachloride
Categories
Funding
- Natural Science Foundation of China [81560626, 81360638]
- Guangxi Natural Science Foundation [2013GXNSFAA019161, 2012GXNSFBA053108]
- Program for Scientific Research in Guangxi Higher Education Institutions [200103YB026]
- Youth Science Foundation of Guangxi Medical University [GXMUYSF13, GXMUYSF18]
- National Training Programs of Innovation and Entrepreneurship for Undergraduates of China [201410598010]
Ask authors/readers for more resources
Liver fibrosis is a common consequence of chronic liver diseases resulting from multiple etiologies. Furthermore, prolonged unresolved liver fibrosis may gradually progress to cirrhosis, and eventually evolve into hepatocellular carcinoma (HCC). Corydalis saxicola Bunting (CS), a type of traditional Chinese folk medicine, has been reported to have hepatoprotective effects on the liver. However, the exact mechanism of how it cures liver fibrosis requires further elucidation. In this work, an integrated approach combining proton nuclear magnetic resonance (1 H-NMR)-based metabonomics and network pharmacology was adopted to elucidate the anti-fibrosis mechanism of CS. Metabonomic study of serum biochemical changes by carbon tetrachloride (CCI4)-induced liver fibrosis in rats after CS treatment were performed using IFI-NMR analysis. Metabolic profiling by means of partial least squares-discriminate analysis (PLSDA) indicated that the metabolic perturbation caused by CC14 was reduced after CS treatment. As a result, lipids, leucine, alanine, acetate, O-acetyl-glycoprotein and creatine were significantly restored after CS treatment, which regulated valine, leucine and isoleucine metabolism; arginine and proline metabolism; lipid metabolism and pyruvate metabolism. Additionally, 157 potential targets of CS and 265 targets of liver fibrosis were identified by means of network pharmacology. Subsequently, 5 target proteins, which are the intersection of potential CS targets and liver fibrosis targets, indicated that CS has potential anti-fibrosis effects through regulating alanine aminotransferase (ALT) activity, the farnesoid X receptor (FXR), cyclooxygenase-2 (COX-2), matrix metalloproteinase-1 (MMP-1) and angiotensinogen. Chelerythrine and sanguinarine were the potential active compounds in CS for treating liver fibrosis through regulating ALT activity. This study is the first report to study the anti-fibrosis effects of CS on the basis of combining a metabonomics and network pharmacology approaches, and it may be a potentially powerful tool to study the efficacy and mechanisms of traditional Chinese folk medicines. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available