Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 150, Issue -, Pages 452-459Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2017.12.008
Keywords
Warfarin-serum albumin interactions; Isothermal titration calorimetry; Fluorescence spectrometry; Frontal analysis capillary electrophoresis
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Funding
- Spanish Government [CTQ2014-56253-P]
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Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding site of human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimental conditions (HEPES buffer 50 mM, pH 7.4 and 25 degrees C) by means of isothermal titration calorimetry (ITC), fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique is based on measurements of a different property of the biochemical system, and then the results allow a critical discussion about the suitability of each approach to estimate the drug-protein binding parameters. The strongest interaction step is properly evaluated by the three assayed approaches being the derived binding constants strongly consistent: from 4 x 10(4) to 7 x 10(4) for HSA and from 0.8 x 10(5) to 1.2 x 10(5) for BSA. Binding enthalpy variations also show consistent results: -5.4 and -5.6 Kcal/mol for HSA and -4.3 and -3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively. Further high order interaction events for both albumins are detected only by FA/CE. (C) 2017 Elsevier B.V. All rights reserved.
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