Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 149, Issue -, Pages 419-424Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2017.11.036
Keywords
Microsampling; Patient centric sampling; Hematocrit; Dried blood spot; Bioanalysis
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Funding
- KTH Royal Institute of Technology
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When using dried blood spot (DBS) sampling for the quantitative bioanalysis of circulating concentrations of drugs, metabolites and endogenous analytes, it is important that a fixed volume of blood is deposited to overcome the issues associated with blood hematocrit (HCT) and homogeneity. The volumetric performance of the KTH DBS collection device was tested with radiolabelled [C-14]-diclofenac. It was demonstrated that the device deposits a fixed volume of blood (13.5 ILL) regardless of the HCT (25-65%), or lipid content of the blood sample. Further, it was found that the precision and accuracy of the derived dried blood samples were at least as good as those of a positive displacement pipette. The device was found to be easy to use and gave acceptable dried samples for 92.9% of the tests performed (n-42). (C) 2017 Elsevier B.V. All rights reserved.
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