Journal
JOURNAL OF PATHOLOGY
Volume 245, Issue 1, Pages 41-52Publisher
WILEY
DOI: 10.1002/path.5054
Keywords
N-glycosylation; basigin; EMMPRIN; CD147; metastasis; protein-protein interaction; epithelial to mesenchymal transition
Funding
- National Basic Research Program of China [2015CB553701]
- National Natural Science Foundation of China [81402383, 31601127]
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While the importance of protein N-glycosylation in cancer cell migration is well appreciated, the precise mechanisms by which N-acetylglucosaminyltransferase V (GnT-V) regulates cancer processes remain largely unknown. In the current study, we report that GnT-V-mediated N-glycosylation of CD147/basigin, a tumor-associated glycoprotein that carries 1,6-N-acetylglucosamine (1,6-GlcNAc) glycans, is upregulated during TGF-1-induced epithelial-to-mesenchymal transition (EMT), which correlates with tumor metastasis in patients with hepatocellular carcinoma (HCC). Interruption of 1,6-GlcNAc glycan modification of CD147/basigin decreased matrix metalloproteinase (MMP) expression in HCC cell lines and affected the interaction of CD147/basigin with integrin 1. These results reveal that 1,6-branched glycans modulate the biological function of CD147/basigin in HCC metastasis. Moreover, we showed that the PI3K/Akt pathway regulates GnT-V expression and that inhibition of GnT-V-mediated N-glycosylation suppressed PI3K signaling. In summary, 1,6-branched N-glycosylation affects the biological function of CD147/basigin and these findings provide a novel approach for the development of therapeutic strategies targeting metastasis. (c) 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
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