Journal
GROWTH FACTORS
Volume 33, Issue 2, Pages 71-78Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/08977194.2015.1013628
Keywords
Bone marrow-derived mesenchymal stem cell; fibroblast growth factor-2; hepatocyte growth factor; L-ascorbic acid 2-phosphate; stemness
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Funding
- Yonsei University Wonju College of Medicine [YUWCM-2005-02]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2012R1A1A2004981]
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L-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess stemness'' during long-term culture.
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