Journal
GREEN CHEMISTRY
Volume 17, Issue 5, Pages 2784-2789Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5gc00422e
Keywords
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Funding
- China Scholarship Council
- State Scholarship Fund
- DOE via Synthetic Design of Microorganisms for Lignin Fuel [EE0006112]
- National Natural Science Foundation of China [51039001, 51378190, 51408206]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT-13R17]
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Kraft lignin (KL) from black liquor is an abundantly available, inexpensive aromatic resource that is regarded as a low value compound by the pulp and paper industry, necessitating the development of new applications. Current work proposes an innovative KL utilization strategy that connects partial lignin degradation with lipid production in oleaginous Rhodococcus. Results showed poor bacterial growth when KL was used directly as a substrate. On the other hand, when KL recalcitrance was reduced by oxygen-pretreatment (O-2-KL), Rhodococcus opacus DSM 1069 was capable of utilizing this material and in turn accumulated lipids. The maximum lipid yield was measured to be 0.067 mg ml(-1) at 36 h of growth and these lipids were mainly composed of palmitic (46.9%) and stearic (42.7%) acids.
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