Journal
THERAPEUTIC DELIVERY
Volume 5, Issue 5, Pages 511-524Publisher
FUTURE SCI LTD
DOI: 10.4155/TDE.14.17
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Funding
- National Natural Science Foundation of China [21071091]
- Shanxi Provincial Natural Science Foundation [2009011012-3]
- Shanxi Scholarship Council of China [201011]
- Shanxi Science and Technology Development Program [20130313021-1]
- Youth Foundation of Shanxi Medical University [02201114]
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Aim: PEGylated fluorescent nanodiamond (FND) conjugated with Tf (FND-PEG-Tf) was investigated for targeted drug delivery. Materials & methods: Human hepatoma (HepG2) and normal (L-02) cell lines were used to investigate the difference in cellular uptake of FND-PEG-Tf and its loading drug system. Nanoparticle uptake was evaluated by flow cytometry and laser scanning confocal microscopy. Results: FNDPEG-Tf showed highly specific TfR-mediated uptake by HepG2 cells, relative to negative controls (L-02 cell), which was a strong correlation among TfR density on the cell surface. The mechanism of TfR-mediated uptake was attested by free Tf with Fe3+ as a competitive agent. The difference in cell viability between L-02 and HepG2 cells treated with doxorubicin hydrochloride (DOX) nanoparticles (FND-PEG-Tf-DOX) can be explained by FND-PEG-Tf, which can target drug delivery to cancer cells. Conclusion: FND-PEG-Tf can potentially be utilized in targeted cancer cell imaging and effective drug delivery for cancer therapy.
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