Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 18, Issue 4, Pages 2286-2293Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2018.14362
Keywords
Solid Phase Gene Delivery; Graphene Oxide; Mesenchymal Stem Cells; Nanofiber Scaffold; PLGA
Categories
Funding
- National Natural Science Foundation of China [81501074]
- Key Research Program of the Chinese Academy of Sciences [ZDRW-ZS-2016-2-3]
- National Basic Research Program [2014CB965003]
- China Postdoctoral Science Foundation [2015M571836]
- Natural Science Foundation of Jiangsu Province [BK20150363]
Ask authors/readers for more resources
Delivery of functional genes into stem cells shows great application prospect in DNA-based tissue engineering. However, comparing with epithelial cells and cancer cells, stem cells usually exhibit low gene transfection efficiency. To enhance the transfection efficiency, non-viral gene delivery in combination with biomaterial scaffolds, has raised increasing interests from researchers in tissue engineering. Nanofibers fabricated by electrospinning technique mimicking extracellular matrix (ECM) are widely used in tissue engineering applications. In addition, graphene oxide (GO) with ultrahigh specific surface area and ultra-strong adsorption capability, is an ideal candidate for gene delivery. In this work, polyethylenimine (PEI)/plasmid DNA-GO/ poly(D,L-lactic-co-glycolic acid) (PLGA) scaffold was developed as a substrate for solid phase gene delivery and a tissue engineering substrate for stem cells growth and differentiation. In order to improve the transfection efficiency of stem cells, PEI/pDNA complexes were immobilized at the surface of electropun GO incorporated PLGA nanofibrous mat. Human embryonic kidney 293 cells and human umbilical cord derived mesenchymal stem cells cultured on PEI/pDNA-GO/PLGA scaffold showed significantly higher green fluorescent protein (GFP) expression than PEI/pGFP in the medium. These findings demonstrated that solid phase gene delivery using PEI/pDNA-GO/PLGA significantly enhanced the gene transfection efficiency, and may find potential application of gene therapy and regeneration medicine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available