Journal
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
Volume 18, Issue 7, Pages 4805-4813Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2018.15304
Keywords
Methanobactin; Direct Electrochemistry; Gold Nanoparticles; Self-Assembled Monolayer; Hydrogen Peroxide
Categories
Funding
- National Natural Science Foundation of China [21573055]
- Research Team Support Project of Harbin University of Commerce [2016TD006]
- Laboratory of Biocatalysis, Food engineering college, Harbin Commerce University of China
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Mathanobatins (Mb, Mbtins) were immobilized successfully on nanometer-sized gold colloid particles associated with beta-mercaptoethylamine. The structures of Mb functionalized gold nanoparticles were characterized and confirmed by UV-vis spectroscopy (UV-vis), FTIR spectra and electrochemical analyses. Direct electron transfer between Mb or copper-loading Mbtins and the modified electrode was investigated without the aid of any electron mediator. The copper-loading Mbtins act as a better electrocatalyst for the reduction of H2O2 than Mb. The copper-loading Mb, with which gold nanoparticles were functionalized, as a model enzyme, was immobilized on gold electrode to construct a novel H2O2 biosensor. In pH 6.4 phosphate buffer solution, the reduction and oxidation peak potentials of Mb functionalized gold nanoparticles modified Au electrode (copper-loading Mbtins) were 0.115 and 0.222 V. On the surface, capacitance per unite area (C-d) of Mb functionalized gold nanoparticles modified electrode were 38 mu F cm(-2). The immobilized Mb displayed the features of a peroxidase and gave an excellent electrocatalytic response to the reduction of H(2)O2(.) The detection limit of Mb functionalized gold nanoparticles (copper-loading) were 09x10(-5) mA/M (S/N = 3). The Michaelis-Menten constant (Km) was 0.787 mM. Good stability and sensitivity were assessed for the biosensor.
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