Journal
JOURNAL OF NANOPARTICLE RESEARCH
Volume 20, Issue 7, Pages -Publisher
SPRINGER
DOI: 10.1007/s11051-018-4286-3
Keywords
Silver nanoparticles; Bovine serum albumin; Protein adsorption; Hard and soft corona; Dynamic light scattering; Fluorescence spectroscopy; Biophysics; Drug delivery
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Funding
- Department of Science and Technology (DST), Government of India, through the DST PURSE-SCHEME, University of Mumbai
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It is well known that when nanoparticles (NPs) are exposed to biological fluid, it results into formation of nanoparticle protein corona, which has been the subject of extensive studies for the development of targeted drug delivery. In this work, we demonstrated the dynamic light scattering, fluorescence, and UV-visible spectroscopy as quantitative and qualitative tools to monitor adsorption of BSA protein onto silver nanoparticles (AgNPs). The adsorption resulted in significant gradual increase in average hydrodynamic radius of BSA-AgNP corona from 24 to 35 nm and its attainment of equilibrium point (saturation) that correlated with albumin concentration enables condition for bound and unbound protein adsorption to be interpreted. Using DLS, the dissociation constant (K-D) was obtained for soft corona to be 2.09 +/- 0.30 mu M. The UV-visible and fluorescence spectroscopy results were correlated with DLS. Loss of percent helicity in secondary structure of adsorbed BSA was monitored in both coronas as compared to native protein. Both coronas were found to be biocompatible with RBC membrane. Further, the results of adsorption isotherm model were used to validate the multilayer formation of albumin protein on silver nanoparticles. The obtained results would be relevant in the drug design development for tumor-targeted therapy.
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