Journal
ACTA SOCIETATIS BOTANICORUM POLONIAE
Volume 83, Issue 1, Pages 67-73Publisher
POLSKIE TOWARZYSTWO BOTANICZNE
DOI: 10.5586/asbp.2014.009
Keywords
callogenesis; extracellular matrix; in vitro culture; scanning electron microscopy; topinambur; transmission electron microscopy
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Funding
- Rector of the Jagiellonian University
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Helianthus tuberosus is economically important species. To improve characters of this energetic plant via genetic modification, production of callus tissue and plant regeneration are the first steps. A new, potentially energetic cultivar Albik was used in this study to test callus induction and regeneration. Callus was produced on leaves, petioles, apical meristems and stems from field-harvested plants but was totally non-morphogenic. Its induction started in the cortex and vascular bundles as confirmed by histological analysis. The surface of heterogeneous callus was partially covered with a membranous extracellular matrix surface network visible in scanning and transmission electron microscopies. The results clearly indicate that: (i) the morphogenic capacity of callus in topinambur is genotype dependent, (ii) cv. Albik of H. tuberosus proved recalcitrant in in vitro regeneration, and (iii) extracellular matrix surface network is not a morphogenic marker in this cultivar.
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