Journal
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
Volume 28, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6439/aaa7a1
Keywords
microfluidics; antibody microarrays; protein extraction; breast cancer tissue; human epidermal growth factor receptor 2 (HER2)
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Funding
- European Union Ideas program [ERC-2012-AdG-320404]
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We report here a new microfluidic method allowing for the quantification of human epidermal growth factor receptor 2 (HER2) expression levels from formalin-fixed breast cancer tissues. After partial extraction of proteins from the tissue slide, the extract is routed to an antibody (Ab) microarray for HER2 titration by fluorescence. Then the HER2-expressing cell area is evaluated by immunofluorescence (IF) staining of the tissue slide and used to normalize the fluorescent HER2 signal measured from the Ab microarray. The number of HER2 gene copies measured by fluorescence in situ hybridization (FISH) on an adjacent tissue slide is concordant with the normalized HER2 expression signal. This work is the first study implementing biomarker extraction and detection from cancer tissue slides using microfluidics in combination with a microarray system, paving the way for further developments towards multiplex and precise quantification of cancer biomarkers.
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