Journal
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
Volume 28, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6439/aaa876
Keywords
stimuli-responsive hydrogel; dynamic crosslink; molecular imprinting; microfluidic device; microchannel; molecular recognition
Categories
Funding
- Japan Society of the Promotion of Science (JSPS) [15H03026, 17K19163]
Ask authors/readers for more resources
Diagnosis sensors using micro-total analysis systems (mu-TAS) have been developed for detecting target biomolecules such as proteins and saccharides because they are signal biomolecules for monitoring body conditions and diseases. In this study, biomolecularly stimuli-responsive micro-sized hydrogels that exhibited quick shrinkage in response to lectin concanavalinA (ConA) were prepared in a microchannel by photopolymerization using a fluorescence microscope. In preparing the micro-size hydrogels, glycosyloxyethyl methacrylate (GEMA) as a ligand monomer was copolymerized with a crosslinker in the presence of template ConA in molecular imprinting. The ConA-imprinted micro-hydrogel showed greater shrinkage in response to target ConA than nonimprinted micro-hydrogel. When a buffer solution was switched to an aqueous ConA solution in the Y-shaped microchannel, the flow rates changed quickly because of the responsive shrinkage of the micro-hydrogel prepared in the microchannel. These results suggest that the ConA-imprinted micro-hydrogel acted as a self-regulated microvalve in microfluidic systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available