A rapid PCR-based method to discriminate Macrococcus caseolyticus and Macrococcus canis from closely-related Staphylococcus species based on the ctaC gene sequence

Our method exploits the amplification of the cytochrome c oxidase subunit II (ctaC) gene for the screening of Macrococcus caseolyticus and Macrococcus canis in complex microbial communities, and discriminating these species from strains of their sister genus Staphylococcus. Thirteen novel strains of these species were isolated using this approach.