Journal
CHEMICAL PAPERS
Volume 68, Issue 8, Pages 1041-1048Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.2478/s11696-014-0558-2
Keywords
coenzyme Q(10); Artemia; extraction; high-performance liquid chromatography
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Funding
- Centre of Excellence for Innovation in Chemistry (PERCH-CIC)
- Office of the Higher Education Commission
- Ministry of Education and Graduate School
- Prince of Songkla University
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A method for extracting coenzyme Q(10) (CoQ(10)) from Artemia was developed. 1 g of fresh Artemia was incubated with 75 % acetic acid at (30 +/- 2)A degrees C for 24 h, followed by three consecutive extractions with a mixture of 5 mL of hexane and 5 mL of ethanol, then analysis by a validated high-performance liquid chromatography with a diode-array detector. The calibration curve for CoQ(10) was linear in a range of 1-50 mu g mL(-1). The limits of detection and quantification were 0.3 mu g mL(-1) and 1.1 mu g mL(-1), respectively. Mean recoveries were 94-100 % with a high precision of below 10 %. The method developed was found to be simple, efficient and the time required for releasing CoQ(10) from Artemia was short. The method provides not only low energy consumption but is also practical for industrial applications.
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