Journal
WATER RESEARCH
Volume 55, Issue -, Pages 304-312Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2014.02.011
Keywords
Microbial community; PCR-DGGE; Domestic wastewater; Constructed wetlands; Biofilters
Funding
- Comissionat per a Universitats i Recerca del Departament d'Innovacio
- Universitats i Empresa de la Generalitat de Catalunya i del Fons Social Europeu
- Ministry of Education and Science of Spain
- Ministry of Science and Innovation of Spain [CTM2008-06676-C05-02/TECNO]
- Consolider TRAGUA [CSD2006-00044, CTQ2009-14390-C02-02]
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The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being gamma-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of alpha, beta and delta-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent. (C) 2014 Elsevier Ltd. All rights reserved.
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