Journal
EUROPEAN CELLS & MATERIALS
Volume 27, Issue -, Pages 237-250Publisher
AO RESEARCH INSTITUTE DAVOS-ARI
DOI: 10.22203/eCM.v027a17
Keywords
Pericellular matrix; human mesenchymal stem cell; type VI collagen; decorin; chondrogenesis; RNA interference
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Funding
- National Science Foundation [CBET-0845754]
- University of Maryland, College Park
- Howard Hughes Medical Institute
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0845754] Funding Source: National Science Foundation
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Human mesenchymal stem cells (hMSCs) induced towards chondrogenesis develop a pericellular matrix (PCM), rich in type VI collagen (ColVI) and proteoglycans such as decorin (DCN). Individual PCM protein functions still need to be elucidated to fully understand the mechanobiological role of this matrix. In this study we identified ColVI and DCN as important contributors in the mechanical function of the PCM and as biochemical modulators during chondrogenesis through targeted knockdown using shRNA lentiviral vectors. Gene expression, western blotting, immunofluorescence and cell deformation analysis were examined at 7, 14 and 28 days post chondrogenic induction. ColVI and DCN knockdown each affected gene expression of acan, bgn, and sox9 during chondrogenesis. ColVI was found to be of central importance in resisting applied strains, while DCN knockdown had strain dependent effects on deformation. We demonstrate that by using genetic engineering to control the biophysical microenvironment created by differentiating cells, it may be possible to guide cellular mechanotransduction.
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