Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 293, Issue -, Pages 82-91Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2018.06.003
Keywords
Photo-CIDNP; Spin-torch; MAS NMR; HETCOR; Hyperpolaritation transfer; Inverse cross polarization; Windowed detection
Funding
- Deutsche Forschungsgemeinschaft [MA-4972/2-1]
- Department of Science and Technology, India [IFA-CH-150]
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In the present study, we exploit the light-induced hyperpolarization occurring on C-13 nuclei due to the solid-state photochemically induced dynamic nuclear polarization (photo-CIDNP) effect to boost the NMR signal intensity of selected protons via inverse cross-polarization. Such hyperpolarization transfer is implemented into H-1-detected two-dimensional C-13-H-1 correlation magic-angle-spinning (MAS) NMR experiment to study protons in frozen photosynthetic reaction centers (RC5). As a first trial, the performance of such an experiment is tested on selectively C-13 labeled RCs from the purple bacteria of Rhodobacter sphaeroides. We observed response from the protons belonging to the photochemically active cofactors in their native protein environment. Such an approach is a potential heteronuclear spin-torch experiment which could be complementary to the classical heteronuclear correlation (HETCOR) experiments for mapping proton chemical shifts of photosynthetic cofactors and to understand the role of the proton pool around the electron donors in the electron transfer process occurring during photosynthesis. (C) 2018 Elsevier Inc. All rights reserved.
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