Journal
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 60, Issue 8, Pages 626-631Publisher
WILEY
DOI: 10.1111/jipb.12667
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Funding
- Chinese Academy of Sciences
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We developed simplified single transcriptional unit (SSTU) CRISPR systems for multiplex gene editing in rice using FnCpf1, LbCpf1 or Cas9, in which the nuclease and its crRNA array are co-expressed from a single Pol II promoter, without any additional processing machinery. Our SSTU systems are easy to construct and effective in mediating multiplex genome editing.
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