Journal
JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
Volume 28, Issue 4, Pages 1624-1635Publisher
SPRINGER
DOI: 10.1007/s10904-018-0838-3
Keywords
Layered double hydroxides; Fe3O4 magnetic fluid; Immobilized cellulase; Layered anchoring structure
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Funding
- National Natural Science Foundation of China [20577010, 20971043]
- Fundamental Research Funds for the Central Universities
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In this study, Fe3O4 nanoparticles were first synthesized by co-precipitation method and then layered double hydroxides (LDHs) nanosheets were anchored onto Fe3O4 via an in-situ growth method to prepare LDHs@Fe3O4 carrier. The obtained magnets and LDHs@Fe3O4 carrier were characterized by XRD, FT-IR, N-2 adsorption, HRSEM and HRTEM respectively. Using glutaraldehyde as a coupling agent, cellulase was immobilized onto this magnetic carrier by covalent binding. The effects of various parameters on enzyme activity of LDHs@Fe3O4 immobilized cellulase, including cellulase concentration, glutaraldehyde concentration, crosslinking time, pH, cellulase concentration, immobilization time and temperature were discussed in detailed. Moreover, thermal stability and operating stability of the immobilized cellulase were studied, the loaded amount of cellulase was measured, and the degradation performance of methoxychlor (MXC) by layered anchoring structure immobilized cellulase was evaluated.
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