Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 454, Issue -, Pages 48-58Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2017.12.003
Keywords
HIV-1 binding antibody response; Microsphere based multiplex antigen binding assay; High-throughput screen
Categories
Funding
- Swiss National Science Foundation (SNF) [314730_152663, 314730_172790]
- Clinical Priority Research Program of the University of Zurich (Viral infectious diseases: Zurich Primary HIV Infection Study)
- Swiss Vaccine Research Institute
- SystemsX.ch grant AntibodyX
- SNF [PZ00P3-142411, BSSGI0_155851, 33CS30_148522]
- small nested SHCS project [744]
- SHCS research foundation
Ask authors/readers for more resources
The monitoring and assessment of a broadly neutralizing antibody (bnAb) based HIV-1 vaccine require detailed measurements of HIV-1 binding antibody responses to support the detection of correlates of protection. Here we describe the development of a flexible, high-throughput rnicrosphere based multiplex assay system that allows monitoring complex binding antibody signatures. Studying a panel of 13 HIV-1 antigens in a parallel assessment of different IgG subclasses (IgG1, IgG2 and IgG3) we demonstrate the potential of our strategy. The technical advances we describe include means to improve antigen reactivity using directed neutravidin-biotin immobilization of antigens and biotin saturation to reduce background. A particular emphasis of our study was to provide tools for the assessment of reproducibility and stability of the assay system and strategies to control for variations allowing the application in high throughput assays, where reliability of single measurements needs to be guaranteed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available