4.3 Article

Induced androgenesis as a biotechnology method for obtaining DH plants in Daucus carota L.

Journal

JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
Volume 93, Issue 6, Pages 625-633

Publisher

TAYLOR & FRANCIS LTD
DOI: 10.1080/14620316.2018.1431058

Keywords

androgenesis; anther culture; carrot; homozygosity; ploidy

Categories

Funding

  1. European Union, Programme Innovative Economy [UDA-POIG.01.03.01-10-114/08-05]

Ask authors/readers for more resources

The influence of various factors on the efficiency of obtaining double haploid lines in anther cultures was examined in Daucus carota L. The impact of the genotype, the formation of donor plants and the growth conditions was investigated. The effects of various regeneration media were analysed. Acclimatisation of androgenic plants and their evaluation were also conducted. Androgenesis was induced on B-5 medium containing 2,4-D and NAA at 0.1g.L-1. Depending on the genotype, 1.2-305.3 embryos per 100 anthers were obtained. The highest number of embryos (5.5 per 100 anthers) was obtained from donor plants with the first-order shoots containing one umbel. Cultivation of donor plants under controlled conditions improved the number of embryos from 305 in the open field to 1764 in the growth chamber. Regeneration of plants from androgenetic embryos proceeded most effectively on B-5 medium without hormones and amino acids. Regenerated plants adapted in over 60%. Ploidy analysis showed the presence of 92% of plants with a doubled chromosome set and 8% with a tetraploid number of chromosomes. Plants with a doubled chromosome set were 73% homozygous for the glucose phosphate isomerase isoenzyme and 100% homozygous for the aspartate aminotransferase isoenzyme, which confirms their gametic origin.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available