Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 66, Issue 11, Pages 813-824Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155418788117
Keywords
animal use alternative; antibodies; antibody specificity; cell culture techniques; epidermis; ichthyosis; skin diseases-genetic; staining and labeling
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Funding
- Fondation du CHU de Quebec
- Quebec Cell, Tissue and Gene Therapy Network-TheCell (a thematic network - Fonds de recherche du Quebec-Sante)
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Lipoxygenases (LOXs) are enzymes likely to be involved in corneocyte lipid envelope formation and skin barrier function. In humans, mutations in epidermis-type lipoxygenase 3 (eLOX-3) and 12R-lipoxygenase (12R-LOX) genes are associated with autosomal recessive congenital ichthyosis (ARCI), whereas deletion of these genes in mice causes epidermal defects. LOXs also represent a matter of interest in psoriasis as well as in cancer research. However, their expression as well as the exact role of these enzymes in normal human skin have not been fully described. Our goal was to characterize the expression of epidermal LOXs in both normal human skin and Tissue-Engineered Skin Substitutes (TESS) and to consider TESS as a potential model for LOX functional studies. Staining for epidermal differentiation markers and LOXs was performed, in parallel, on normal human skin and TESS. Our results showed similar expression profiles in TESS when compared with native skin for e-LOX3, 12R-LOX, 12S-lipoxygenase (12S-LOX), and 15-lipoxygenase 2 (15-LOX-2) but not for 15-lipoxygenase 1 (15-LOX-1). Because of their appropriate epidermal differentiation and LOX expression, TESS represent an alternative model for future studies on LOX function.
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