Journal
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
Volume 42, Issue 7, Pages 942-947Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1872-2040(14)60750-7
Keywords
Aptamer; Pb2+ sensor; Electrochemiluminescence; Quantum dots; Turn on model; G-quadruplex
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Funding
- National Natural Science Foundation of China [21165007, 21375031]
- Experiment Center of Mining, Metallurgy and Environmental Science of Guangxi Province of China [KH20121YB003]
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A turn on model of electrochemiluminescence Pb2+ biosensor based on G-quadruplex of aptamer probe was developed by using quantum dots as labels. The biosensor was prepared by immobilizing a hairpin aptamer probe with a thiol group at 5'-end and an amino group at 3'-end on gold electrode via an Au-S bond. In the presence of Pb2+, the G-rich hairpin aptamer probe opens the stem-loop and forms G-quadruplex, which exposes the amino group at 3'-end that could covalently link to CdS quantum dots with carboxyl group on the surface. The fabrication and reaction process of the Pb2+ biosensor were characterized. The responsive electrochemiluminescence intensity was linearly related to the Pb2+ concentrations in the range of 2.0 x 10(-10)-5.0 x 10(-8) M, with a detection limit of 3.74 x 10(-11) M. The ECL biosensor has high sensitivity, good selectivity, satisfying stability and repeatability.
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