Journal
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Volume 64, Issue 3, Pages 127-135Publisher
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.2017.11.001
Keywords
interspecies hybrid; mating; mating-type-like (MTL) loci; Zygosaccharomyces
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Funding
- Miso Central Research
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Variations of chromosomal structures and nucleotide sequences around mating-type-like (MTL) loci among Zygosaccharomyces species have been reported. We have analyzed these differences in more detail and, on the basis of PCR- and next-generation sequencing data, we describe the MTL loci on chromosomes C and F for Z. rouxii type-strain NBRC1130, Z. rouxii N It 12 C0740 and Zygosaccharomyces sp. NBRC 1876. We developed a mating strategy for Zygosaccharomyces sp. NBRC1876 and Z. rouxii NBRC0740, and found that the mated stains could be identified from parental strains on the basis of nucleotide sequence variations of the MTL loci. We further obtained evidence that Zygosaccharomyces sp. NBRC1876 is a natural interspecies hybrid between Z. rouxii and a related species.
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