Journal
JOURNAL OF EXPERIMENTAL BOTANY
Volume 69, Issue 7, Pages 1749-1764Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ery020
Keywords
Abiotic stress; Chinese wild grape; cold response; galactinol synthase; grape transformation; HRS1; low temperature exotherms; raffinose; ROS
Categories
Funding
- Youth Innovation Promotion Association of CAS [2015281]
- China Postdoctoral Science Foundation [2016M601166]
- Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025]
- Grape Breeding Project of Ningxia [NXNYYZ201502]
Ask authors/readers for more resources
Grapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera, and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and in Amur grape calli. In Arabidopsis, the ectopic expression of VaAQ increased antioxidant enzyme activities and up-regulated reactive oxygen species-(ROS) scavenging-related genes. Comparative mRNA sequencing profiling of 35S: VaAQ Arabidopsis plants suggests that this transcription factor is related to phosphate homeostasis like their Arabidopsis closest homologues: AtHRS1 and AtHHO2. However, when a cold stress is imposed, AQ is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GoLS) and raffinose synthase genes. Gene co-expression network (GCN) and cis-regulatory element (CRE) analyses in grapevine indicated AQ as potentially regulating VvGoLS genes. Increased RFO content was confirmed in both transgenic Arabidopsis and Amur grape calli overexpressing VaAQ. Taken together, our results imply that AQ improves cold tolerance through promoting the accumulation of osmoprotectants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available